DEEP, however, allows for wide-scale and quick tissue penetration, and produces high-resolution images. The system projects a wide light into the subject as in the temporal microscopy method, but that laser light is in a specific pattern. The computational-imaging algorithm that knows the initial pattern takes in the information gathered to reverse the process when it gets scattered and then reconstructs it, de-scattering the image. This is especially notable since it takes the reconstruction of structural features from millions of measurements to tens and hundreds. DEEP can image hundreds of microns deep through scattering tissue comparable to point-scanning techniques.

DEEP is still in its early years of development, but is emerging from its proof-of-concept phase.

“We showed that we can image about 300 microns into the brains of live mice,” Wadduwage said. “But since this is only the first demonstration, almost all aspects of the technique have room for improvement.”